| 產(chǎn)品編號 | bs-1617R |
| 英文名稱 | Rabbit Anti-CDH17／LI Cadherin antibody |
| 中文名稱 | 肝腸鈣粘連蛋白抗體 |
| 別 名 | CAD17_HUMAN; Cadherin-17; CDH17; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin (LI-cadherin); HPT1; CDH16; HPT-1; |
| 研究領(lǐng)域 | 免疫學 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LI-cadherin: 101-200/832 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009] Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport. Subcellular Location: Cell membrane; Single-pass type I membrane protein (Potential). Tissue Specificity: Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin. Similarity: Contains 7 cadherin domains. SWISS: Q12864 Gene ID: 1015 Database links: Entrez Gene: 1015 Human Omim: 603017 Human SwissProt: Q12864 Human Unigene: 591853 Human 肝腸鈣粘連蛋白是鈣粘連蛋白超家族中的特殊一員,在結(jié)構(gòu)上與經(jīng)典Ⅰ型鈣粘連蛋白有所不同而被劃分為經(jīng)典鈣粘連蛋白的變異體，主要用于消化道腫瘤方面的研究。 |
| 產(chǎn)品圖片 |
Sample:
SW480(Human) Cell Lysate at 30 ug
Primary: Anti-LI Cadherin (bs-1617R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 130 kD
Sample:
Lane 1: Human FHC cell Lysates
Lane 2: Human LOVO cell Lysates
Lane 3: Human SW480 cell Lysates
Primary: Anti-LI Cadherin(bs-1617R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90kDa
Observed band size: 130kDa
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
