| 產(chǎn)品編號(hào) | bs-1464R |
| 英文名稱 | Rabbit Anti-GnRHR antibody |
| 中文名稱 | 促性腺激素釋放激素受體抗體 |
| 別 名 | Gonadotropin-releasing hormone receptor; GH1; Lhrhr; GnRH receptor; gnrh-r; GnRHR; GNRHR1; Gonadotropin releasing hormone receptor; GRHR; leutinizing-releasing hormone receptor; lh-rh; LHRHR; LRHR; luteinizing hormone releasing hormone receptor; GNRHR_HUMAN |
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Specific References (4) | bs-1464R has been referenced in 4 publications.
[IF=4.736] Lu Xiaosheng. et al. Deficiency of C1QL1 reduced murine ovarian follicle reserve through intraovarian and endocrine control. ENDOCRINOLOGY. 2022 Apr;: IHC ; Mouse.
[IF=3.34] López-Doval, S., R. Salgado, and A. Lafuente. "The expression of several reproductive hormone receptors can be modified by perfluorooctane sulfonate (PFOS) in adult male rats." Chemosphere 155 (2016): 488-497. Rat.
[IF=3.14] Liu, Te, et al. "Effects of di-(2-ethylhexyl) phthalate on the hypothalamus-pituitary-ovarian axis in adult female rats." Reproductive Toxicology (2014). IHC-P ; Rat.
[IF=1.39] Xiao, Liu, et al. "Distribution patterns and developmental changes of GnRH and GnRHR-immunopositive cells in the pituitary of Ji Ning gray goats." Pakistan Veterinary Journal 34.1 (2014): 116-119. IHC-P ; Goat.
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| 研究領(lǐng)域 | 信號(hào)轉(zhuǎn)導(dǎo) 生長(zhǎng)因子和激素 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GnRHR: 201-328/328 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Gonadotropin Releasing Hormone (GnRH) is down-regulated by hCG and believed to be an autocrine factor that regulates the ovary. The Gonadotropin Releasing Hormone Receptor (GnRHR) is synthesized in the pituitary gland. Activin A has been shown to stimulate the synthesis of GnRHR, illustrating a possible mechanism for the modulation of gonadotropin responsiveness to GnRH. Function: Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Pituitary, ovary, testis, breast and prostate but not in liver and spleen. DISEASE: Hypogonadotropic hypogonadism 7 with or without anosmia (HH7) [MIM:146110]: A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no other abnormalities of the hypothalamic-pituitary axis. In some cases, it is associated with non-reproductive phenotypes, such as anosmia, cleft palate, and sensorineural hearing loss. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In the presence of anosmia, idiopathic hypogonadotropic hypogonadism is referred to as Kallmann syndrome, whereas in the presence of a normal sense of smell, it has been termed normosmic idiopathic hypogonadotropic hypogonadism (nIHH). Note=The disease is caused by mutations affecting the gene represented in this entry. Fertile eunuch syndrome (FEUNS) [MIM:228300]: Mild phenotypic form of HH going with the presence of normal testicular size and some degree of spermatogenesis. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P30968 Gene ID: 2798 Database links: Entrez Gene: 2798 Human Entrez Gene: 100009509 Rabbit Omim: 138850 Human SwissProt: P30968 Human Unigene: 407587 Human 信號(hào)傳導(dǎo)（Signaling Intermediates） Grb2是生長(zhǎng)因子受體結(jié)合蛋白2,又稱Ash蛋白。該蛋白參與細(xì)胞內(nèi)各種受體激活后的下游調(diào)節(jié)。能夠直接與激活的表皮生長(zhǎng)因子受體磷酸化的酪氨酸結(jié)合,參與EGF受體介導(dǎo)的信號(hào)轉(zhuǎn)導(dǎo), 屬SH蛋白. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-GnRHR (bs-1464R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62-70 kD
Observed band size: 63 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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