| 產(chǎn)品編號 | bs-1334R |
| 英文名稱 | Rabbit Anti-TIE1 antibody |
| 中文名稱 | 血管生成素受體1抗體 |
| 別 名 | TIE1_HUMAN; JKT 14; JTK14; TIE; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains; Tyrosine protein kinase receptor Tie 1; Tyrosine-protein kinase receptor Tie-1; tyrosine kinase receptor 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1. |
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Specific References (1) | bs-1334R has been referenced in 1 publications.
[IF=5.57] Zhang C et al. Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats. Nanomedicine. 2019 Aug 7;21:102078. IHC-P&WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 心血管 信號轉(zhuǎn)導(dǎo) 干細胞 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 123kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Tie1: 851-1000/1138 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium. Function: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Subunit: Heterodimer with TEK/TIE2. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Specifically expressed in developing vascular endothelial cells. Post-translational modifications: Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. Contains 3 EGF-like domains. Contains 3 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain. SWISS: P35590 Gene ID: 7075 Database links: Entrez Gene: 7075 Human Entrez Gene: 21846 Mouse Omim: 600222 Human SwissProt: P35590 Human SwissProt: Q06806 Mouse Unigene: 78824 Human Unigene: 4345 Mouse Unigene: 13171 Rat 血管生成素-1受體又稱上皮生長因子樣域酪氨酸激酶1或內(nèi)皮酪氨酸激酶受體,Tie1在血管內(nèi)皮細胞、腺上皮及間質(zhì)細胞中均有表達，主要定位于細胞質(zhì)。 |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-TIE1 (bs-1334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123 kD
Observed band size: 123 kD
Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TIE1 Polyclonal Antibody, Unconjugated(bs-1334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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