| 產(chǎn)品編號 | bs-0628R |
| 英文名稱 | Rabbit Anti-ZNF300 antibody |
| 中文名稱 | 鋅指蛋白300抗體 |
| 別 名 | Kruppel like zinc finger protein; Zinc finger protein 300; ZN300_HUMAN. |
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Specific References (1) | bs-0628R has been referenced in 1 publications.
[IF=1.291] Xue et al. ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site. (2010) Cell.Mol.Biol.Lett. 15:530-40 WB ;
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| 研究領域 | 染色質和核信號 信號轉導 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human) |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF300: 165-250/604 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms. Function: Has a transcriptional repressor activity. Subcellular Location: Nucleus. Tissue Specificity: Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 12 C2H2-type zinc fingers. Contains 1 KRAB domain. SWISS: Q96RE9 Gene ID: 91975 Database links: Entrez Gene: 91975 Human Omim: 612429 Human SwissProt: Q96RE9 Human Unigene: 134885 Human ZNF300白血病相關性研究。 |
| 產(chǎn)品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-ZNF300 (bs-0628R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Sample:
Lane1: Testis (Mouse) Lysate at 30 ug
Lane2: Small intestine (Mouse) Lysate at 30 ug
Primary: Anti-ZNF300(bs-0628R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution;
Predicted band size : 66kD
Observed band size : 70,66kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
