| 產(chǎn)品編號 | bs-1212R |
| 英文名稱 | Rabbit Anti-TRAF1 antibody |
| 中文名稱 | 腫瘤壞死因子受體相關(guān)因子1抗體 |
| 別 名 | TNF receptor-associated factor 1; 4732496E14Rik; EBV induced protein 6; Epstein-Barr virus-induced protein 6; Tnf receptor associated factor 1; TRAF 1; TRAF1_HUMAN. |
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Specific References (2) | bs-1212R has been referenced in 2 publications.
[IF=3.3] Wang et al. Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori. (2012) World.J.Gastroenterol. 18:7212-24 IHC ; Human,?Mouse,?Rat,?Dog,?Cow,?Horse,?Rabbit.
[IF=2.674] Yao Zheng. et al. Comparative proteomic analysis of spleen reveals key immune-related proteins in the yak (Bos grunniens) at different growth stages. Comp Biochem Phys D. 2022 Jun;42:100968 IHC ; Yak.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRAF1: 301-416/416 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. [provided by RefSeq]. Function: Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. Subunit: Homotrimer. Heterotrimer with TRAF2. Interacts with TNFRSF1A/TNFR1, TNFRSF1B/TNFR2, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13C, TNFRSF18/AITR, TNFRSF17/BCMA, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1, TANK/ITRAF, TRAIP and RIPK2. Interacts with BIRC2 and BIRC3 N-terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2. Interacts with NFATC2IP, TRAFD1 and with HIVEP3 (By similarity). Interacts with MAP3K14. Post-translational modifications: Polyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation. Similarity: Contains 1 MATH domain. SWISS: Q13077 Gene ID: 7185 Database links: Entrez Gene: 7185 Human Entrez Gene: 22029 Mouse Omim: 601711 Human SwissProt: Q13077 Human SwissProt: P39428 Mouse Unigene: 531251 Human Unigene: 239514 Mouse 信號傳導(dǎo)（Signaling Intermediates） TRAF1是TRAFs分子中最有可能出現(xiàn)差異表達(dá)的分子之一；TRAF1分子不包含其它TRAFs分子皆有的環(huán)結(jié)構(gòu)-沒有重排的鋅指結(jié)構(gòu)。TNFR1在介導(dǎo)細(xì)胞凋亡信號及在腫瘤細(xì)胞的凋亡中發(fā)揮著一定的作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAF1) Polyclonal Antibody, Unconjugated (bs-1212R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAF1) Polyclonal Antibody, Unconjugated (bs-1212R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAF1 Polyclonal Antibody, Unconjugated(bs-1212R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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