| 產(chǎn)品編號 | bs-1196R |
| 英文名稱 | Rabbit Anti-PAR4 antibody |
| 中文名稱 | 蛋白酶活化受體4抗體 |
| 別 名 | F2RL3; F2R Like Thrombin/Trypsin Receptor 3; Coagulation Factor II (Thrombin) Receptor-Like 3; Thrombin Receptor-Like 3; PAR-4; Coagulation Factor II Receptor-Like 3; Proteinase-Activated Receptor-4; Proteinase-Activated Receptor 4; Protease-Activated Receptor-4; PAR4_HUMAN; |
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Specific References (1) | bs-1196R has been referenced in 1 publications.
[IF=3.61] Oláh, Zita, et al. "Proteomic Analysis of Cerebrospinal Fluid in Alzheimers Disease: Wanted Dead or Alive." Journal of Alzheimers Disease (2014). WB ; Human.
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| 研究領域 | 細胞生物 信號轉(zhuǎn)導 激酶和磷酸酶 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Proteinase-activated receptor 4: 301-385/385 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. [provided by RefSeq, Jul 2008] Function: Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. Post-translational modifications: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q96RI0 Gene ID: 9002 Database links: Entrez Gene: 9002 Human Omim: 602779 Human SwissProt: Q96RI0 Human Unigene: 137574 Human
蛋白酶活化受體-4是蛋白酶活化受體PARs (protease-activated receptors , PARs)的成員之一，PAR4在激活、滅活、脫敏、復敏、及其與信號轉(zhuǎn)導途徑的關系,尤其是與疾病的關系正倍受關注。 PAR4具有廣泛的生物學效應,最終影響細胞的形態(tài)的改變、分泌蛋白和整合蛋白的活化、代謝反應、轉(zhuǎn)錄過程和細胞運動，由此參與細胞的信號轉(zhuǎn)導作用。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Lung tissue lysates
Lane 4: Rat Thyroid gland tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Human K562 cell lysates
Lane 7: Human THP-1 cell lysates
Lane 8: Human Hela cell lysates
Lane 9: Human Huvec cell lysates
Primary: Anti-Proteinase-activated receptor 4/PAR4 (bs-1196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41/38 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAR4 Polyclonal Antibody, Unconjugated(bs-1196R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
