| 產(chǎn)品編號 | bs-0006R |
| 英文名稱 | Rabbit Anti-Nestin antibody |
| 中文名稱 | 巢蛋白/神經(jīng)上皮干細胞蛋白抗體 |
| 別 名 | ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin. |
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Specific References (2) | bs-0006R has been referenced in 2 publications.
[IF=1.405] Minyun Chen. et al. Retinoic acid induces differentiation of cochlear neural progenitor cells into hair cells. Braz J Otorhinolar. 2021 Feb;: IF,ICC ; Mouse.
[IF=1.29] Fan, Lixing, et al. "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons."?In Vitro Cellular & Developmental Biology-Animal?(2013): 1-9. Human.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 發(fā)育生物學 神經(jīng)生物學 干細胞 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 178kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nestin: 801-900/1621 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
There are a certain number of neural stem cells, which can be potentially differentiated into the neurons or neuroglia, in the adult mammalian brain. These neural precursors are located in the subependymal cell layer of the ventricle. Those precursors isolated from embryonic brain can be divided and differentiated into the neurons and glia in vitro and this is regulated by a series of growth-factor actions. Function: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Subunit: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 (By similarity). Tissue Specificity: CNS stem cells. Post-translational modifications: Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Similarity: Belongs to the intermediate filament family. SWISS: P48681 Gene ID: 10763 Database links: Entrez Gene: 10763 Human Entrez Gene: 18008 Mouse Omim: 600915 Human SwissProt: P48681 Human SwissProt: Q6P5H2 Mouse Unigene: 527971 Human Unigene: 331129 Mouse Unigene: 9701 Rat
結(jié)構(gòu)蛋白（Structural Proteins） Neural Stem Cell Marker (神經(jīng)干細胞標志物) 在動物體早期發(fā)育過程中，中樞神經(jīng)系統(tǒng)發(fā)育尤為重要 。Nestin是一種中等纖維蛋白，它在哺乳動物神經(jīng)前體細胞中表達較高，目前已被廣泛用于神經(jīng)前體細胞的重要標志分子。研究Nestin的表達調(diào)控規(guī)律，將有助于人們認識神經(jīng)前體細胞的生物特性。此抗體還表達中樞神經(jīng)系統(tǒng)干細胞及神經(jīng)上皮細胞。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nestin) Polyclonal Antibody, Unconjugated (bs-0006R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nestin) Polyclonal Antibody, Unconjugated (bs-0006R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: The human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0006R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): U251 (fixed with 2% paraformaldehyde (10 min)and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-Nestin antibody (bs-0006R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
