| 產(chǎn)品編號 | bs-0815R |
| 英文名稱 | Rabbit Anti-Megsin antibody |
| 中文名稱 | 絲氨酸蛋白酶抑制劑B7抗體 |
| 別 名 | SER-PINB7; MEGSIN; Mesangium predominant gene megsin; MGC120014; MGC120015; Serpin B7; Serpin peptidase inhibitor clade B (ovalbumin) member 7; TP55; SPB7_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細胞凋亡 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Megsin: 301-380/380 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Serpin B7 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in a search for agents responsible for thrombopoiesis, and maturation of megakaryocytes. SerpinB7 was identified as a protein that increased acetylcholine esterase stimulation from bone barrow progenitor cells, and injection of SerpinB7 in mice induced a 40% increase in platelets compared to controls. Shortly thereafter, in a search for kidney specific genes, SerpinB7 was described as a protein specific to mesangial cells, and was named megsin to indicate the tissue specificity of the protein. Function: Might function as an inhibitor of Lys-specific proteases. Might influence the maturation of megakaryocytes via its action as a serpin. Subcellular Location: Cytoplasm. Tissue Specificity: Predominantly expressed in mesangial cells. Similarity: Belongs to the serpin family. Ov-serpin subfamily. SWISS: O75635 Gene ID: 8710 Database links: Entrez Gene: 8710 Human Omim: 603357 Human SwissProt: O75635 Human Unigene: 138202 Human Unigene: 742388 Human megsin是新近發(fā)現(xiàn)的蛋白質(zhì),屬于絲氨酸蛋白酶抑制劑（Serpin）超家族，特異表達于腎小球系膜細胞,其表達異常在腎臟疾病進展中具有重要作用,megsin蛋白適量的表達，對維持人體正常的生理病理過程如凝血、纖維溶解、間質(zhì)代謝、發(fā)育、血壓、腫瘤和炎癥等是很重要的。在系膜細胞增生和系膜基質(zhì)增多明顯的IgA腎病腎小球中，megsin的表達顯著上調(diào)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Ear (Rat) Lysate at 40 ug
Lane 2: Tongue (Rat) Lysate at 40 ug
Primary: Anti-Megsin (bs-0815R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin ) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin ) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Megsin Polyclonal Antibody, Unconjugated(bs-0815R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
