| 產(chǎn)品編號(hào) | bs-0792R |
| 英文名稱 | Rabbit Anti-Maspin antibody |
| 中文名稱 | 抑癌基因抗體 |
| 別 名 | mammary serine proteinase inhibitor; Maspin; Ovalbumin; PI-5; PI5; Protease inhibitor 5; Serine (or cysteine) peptidase inhibitor, clade B, member 5; Serpin B5; serpin peptidase inhibitor; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; SERPINB5; SPB5_HUMAN; Spi5; Spi7. |
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Specific References (1) | bs-0792R has been referenced in 1 publications.
[IF=1.48] Sun, Pengming, et al. "Expression patterns of maspin and mutant p53 are associated with the development of gestational trophoblastic neoplasia."Oncology Letters (2016). IHC-P ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Maspin: 301-375/375 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Maspin protein is a sour depressant of nenzyme of a kind of silk ammonia that represses the tumor, is the sour depressant of protei nenzyme one member within super household of the silk ammonia, it is in repress the tumor growth and transfer exertive certain function. Function: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Subunit: Interacts with IRF6. Subcellular Location: Secreted, extracellular space. Tissue Specificity: Normal mammary epithelial cells. Similarity: Belongs to the serpin family. Ov-serpin subfamily. SWISS: P36952 Gene ID: 5268 Database links: Entrez Gene: 5268 Human Entrez Gene: 20724 Mouse Omim: 154790 Human SwissProt: P36952 Human SwissProt: P70124 Mouse Unigene: 55279 Human Unigene: 268618 Mouse Unigene: 25752 Rat Maspin蛋白是一種抑制腫瘤的絲氨酸蛋白酶抑制劑, 是絲氨酸蛋白酶抑制劑超家族中的一員，它在抑制腫瘤生長(zhǎng)和轉(zhuǎn)移中發(fā)揮一定作用。 |
| 產(chǎn)品圖片 |
Sample:
MDA-MB-231(Human) Cell Lysate at 40 ug
Primary: Anti-Maspin (bs-0792R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lane 1: Mouse Skin Lysates
Lane 2: Rat Skin Lysates
Primary: Anti-Maspin (bs-0792R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kDa
Observed band size: 42kDa
Tissue/cell: Rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(bs-0792R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(bs-0792R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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