| 產(chǎn)品編號 | bs-1054R |
| 英文名稱 | Rabbit Anti-HDC antibody |
| 中文名稱 | L-組氨酸脫羧酶抗體 |
| 別 名 | L-Histidine decarboxylase; EC 4.1.1.22; HDC; L-HDC; Histidine decarboxylase; DCHS_HUMAN. |
| 研究領域 | 免疫學 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HDC: 351-450/662 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Histidine decarboxylase(HDC) is the enzyme that calalyses the conversion of histidine into histamine. It can be found in the histamine secreting ECL cells of some species, as well as in the mast cells.The biogenic amine histamine is an important modulator of numerous physiological processes, including neurotransmission, gastric acid secretion and smooth muscle tone. Function: Catalyzes the biosynthesis of histamine from histidine. Subunit: Homodimer. Similarity: Belongs to the group II decarboxylase family. SWISS: P19113 Gene ID: 3067 Database links: Entrez Gene: 3067 Human Entrez Gene: 15186 Mouse Omim: 142704 Human SwissProt: P19113 Human SwissProt: P23738 Mouse Unigene: 1481 Human Unigene: 18603 Mouse Unigene: 48653 Rat
主要用于多種變態(tài)反應及炎性浸潤改變有關的研究。 |
| 產(chǎn)品圖片 |
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HDC Polyclonal Antibody, Unconjugated(bs-1054R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
