| 產(chǎn)品編號 | bs-1109R |
| 英文名稱 | Rabbit Anti-IQGAP1 antibody |
| 中文名稱 | 支架蛋白抗體 |
| 別 名 | IQ motif containing GTPase activating protein 1; KIAA0051; p195; Ras GTPase activating like protein 1; IQGA1_HUMAN. |
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Specific References (2) | bs-1109R has been referenced in 2 publications.
[IF=5.942] Sheen YS et al. Purpuric drug eruptions induced by EGFR tyrosine kinase inhibitors are associated with IQGAP1‐mediated increase in vascular permeability. J Pathol. 2020 Feb 7. ICF ; Human.
[IF=4.67] Fang, Jing, et al. "PAX6 down-regulates miR-124 expression to promote cell migration during embryonic stem cell differentiation." Stem Cells and Development (2014). Mouse.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞凋亡 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat,Rabbit (predicted: Mouse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 189kDa |
| 細胞定位 | 細胞核 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IQGAP1: 1451-1550/1657 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
IQGAP1 is a widely expressed protein than interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology. It binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Function: Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Subunit: Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Subcellular Location: Cell membrane. Tissue Specificity: Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas. Post-translational modifications: Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. Similarity: Contains 1 CH (calponin-homology) domain. Contains 4 IQ domains. Contains 1 Ras-GAP domain. Contains 1 WW domain. SWISS: P46940 Gene ID: 8826 Database links: Entrez Gene: 8826 Human Entrez Gene: 29875 Mouse Omim: 603379 Human SwissProt: P46940 Human SwissProt: Q9JKF1 Mouse Unigene: 430551 Human Unigene: 207619 Mouse Unigene: 106939 Rat 支架蛋白IQGAP1與許多重要的細胞活動緊密相關，如黏附和遷移。研究發(fā)現(xiàn)在損傷修復、組織發(fā)展、腫瘤轉移等過程中，IQGAP1的表達和功能有明顯改變。同時作為Rh.GTPase信號的主要效應因子，IQGAP1能傳遞細胞外信號至黏附素復合體、細胞骨架以及細胞核。通過上述信號通路，IQ-GAP1協(xié)助細胞完成一系列重要生理病理活動。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IQGAP1 Polyclonal Antibody, Unconjugated(bs-1109R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rabbit placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IQGAP1 Polyclonal Antibody, Unconjugated(bs-1109R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-IQGAP1 antibody (bs-1109R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
