| 產(chǎn)品編號 | bs-0421R |
| 英文名稱 | Rabbit Anti-DR3 antibody |
| 中文名稱 | 死亡受體3抗體 |
| 別 名 | Apo 3; Apo3; Apo-3; Apoptosis inducing receptor AIR; Apoptosis inducing receptor; Apoptosis mediating receptor; Apoptosis mediating receptor DR3; Apoptosis mediating receptor TRAMP; DDR 3; DDR3; Death domain receptor 3; Death Receptor 3; Death receptor beta; DR 3; LARD; Lymphocyte associated receptor of death; TNFRSF 12; TNFRSF 25; TNFRSF12; TNFRSF25; TR 3; TR3; TRAMP; Translocating chain association membrane protein; Tumor necrosis factor receptor superfamily member 12; Tumor necrosis factor receptor superfamily member 25; WSL 1; WSL; WSL LR; WSL1; WSL1 protein; WSLLR; TNR25_HUMAN. |
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Specific References (1) | bs-0421R has been referenced in 1 publications.
[IF=3.71] ?lebioda, Tomasz Jerzy, et al. "TL1A as a potential local inducer of IL17A Expression in colon mucosa of inflammatory bowel disease patients."Scandinavian Journal of Immunology (2015). IHC-P ; Human.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DR3: 351-417/417 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008] Subunit: Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 9: Cell membrane; Single-pass type I membrane protein. Isoform 11: Cell membrane; Single-pass type I membrane protein. Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Secreted. Isoform 6: Secreted. Isoform 7: Secreted. Isoform 8: Secreted. Isoform 10: Secreted. Isoform 12: Secreted. Tissue Specificity: Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. Similarity: Contains 1 death domain. Contains 4 TNFR-Cys repeats. SWISS: Q93038 Gene ID: 8718 Database links: Entrez Gene: 8718 Human Entrez Gene: 85030 Mouse Omim: 603366 Human SwissProt: Q93038 Human Unigene: 462529 Human Unigene: 101198 Mouse Unigene: 106560 Rat 死亡受體(death receptor)已經(jīng)鑒定的有DR3、DR4和DR5，DR3也稱為WSL-1,Apo-3受體或TRAMP(TNFR介導(dǎo)的細胞凋亡蛋白質(zhì)TNF-receptor apoptosis-mediating protein，TRAMP)。 腫瘤壞死因子-α受體(腫瘤壞死因子受體超家TNF-α膜受體族成員)是近年來被鑒定發(fā)現(xiàn)的：主要在人的多淋巴細胞組織中。它們同屬TNF／NGF受體超家族成員。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Thymus (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Mouse) Lysate at 40 ug
Lane 4: Thymus (Rat) Lysate at 40 ug
Primary: Anti-DR3 (bs-0421R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DR3) Polyclonal Antibody, Unconjugated (bs-0421R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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