| 產(chǎn)品編號 | bs-0301R |
| 英文名稱 | Rabbit Anti-Dnmt3b antibody |
| 中文名稱 | DNA甲基轉(zhuǎn)移酶-3β抗體 |
| 別 名 | Cytosine 5methyltransferase 3B; DNA (cytosine 5) methyltransferase 3 beta; DNA; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; Dnmt3b; Dnmt3 Beta; EC 2.1.1.37; ICF; M.HsaIIIB; MGC124407; RP23-89H14.3; DNM3B_HUMAN. |
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Specific References (5) | bs-0301R has been referenced in 5 publications.
[IF=8.739] Ai Wei. et al. Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis. 2021 Aug 11 WB,IHC ; Mouse.
[IF=5.2] Zhao, Qian, et al. "Prenatal Cocaine Exposure Impairs Cognitive Function of Progeny Via Insulin Growth Factor II Epigenetic Regulation." Neurobiology of Disease (2015). WB ; Mouse.
[IF=3.85] Wu, Yuting, et al. "Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis." Toxicology and Applied Pharmacology 315 (2017): 35-49. WB ; Mouse.
[IF=3.699] Xiao-Tan Zhang . et al. Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model. Cell Cycle. 2020;19(23):3329-3347 WB ; Mouse.
[IF=2.976] Huang et al. Expression of DNA methyltransferases and target microRNAs in human tissue samples related to sporadic colorectal cancer. (2016) Oncol.Rep. 36:2705-2714 IHC/WB ; human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 94kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Dnmt3 Beta: 1-80/853 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. Function: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. Subunit: Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. Post-translational modifications: Sumoylated. DISEASE: Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome type 1 (ICF1) [MIM:242860]. A rare disorder characterized by a variable immunodeficiency, facial anomalies, and branching of chromosomes 1, 9, and 16. Other variable symptoms include growth retardation, failure to thrive, and psychomotor retardation. Laboratory studies show limited hypomethylation of DNA in a small fraction of the genome in some, but not all, patients. Similarity: Belongs to the C5-methyltransferase family. Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain. SWISS: Q9UBC3 Gene ID: 1789 Database links: Entrez Gene: 1789 Human Entrez Gene: 13436 Mouse Omim: 602900 Human SwissProt: Q9UBC3 Human SwissProt: O88509 Mouse Unigene: 643024 Human Unigene: 713611 Human Unigene: 89772 Mouse Unigene: 117353 Rat 腫瘤組織存在DNA甲基化紊亂，包括與細(xì)胞增殖周期密切相關(guān)的癌基因低甲基化和抑癌基因高甲基化DNA甲基轉(zhuǎn)移酶(Dnmt)參與甲基化的形成(主要是Dnmt3a和Dnmt3b)和維持（主要是Dnmt1)。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dnmt3b) Polyclonal Antibody, Unconjugated (bs-0301R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dnmt3b) Polyclonal Antibody, Unconjugated (bs-0301R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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